- Research Article
- Open Access
Pancreatic Expression and Mitochondrial Localization of the Progestin-AdipoQ Receptor PAQR10
Molecular Medicine volume 14, pages697–704(2008)
Steroid hormones induce changes in gene expression by binding to intracellular receptors that then translocate to the nucleus. Steroids have also been shown to rapidly modify cell function by binding to surface membrane receptors. We identified a candidate steroid membrane receptor, the progestin and adipoQ receptor (PAQR) 10, a member of the PAQR family, in a screen for genes differentially expressed in mouse pancreatic β-cells. PAQR10 gene expression was tissue restricted compared with other PAQRs. In the mouse embryonic pancreas, PAQR10 expression mirrored development of the endocrine lineage, with PAQR10 protein expression confined to endocrine islet-duct structures in the late embryo and neonate. In the adult mouse pancreas, PAQR10 was expressed exclusively in islet cells except for its reappearance in ducts of maternal islets during pregnancy. PAQR10 has a predicted molecular mass of 29 kDa, comprises seven transmembrane domains, and, like other PAQRs, is predicted to have an intracellular N-terminus and an extracellular C-terminus. In silico analysis indicated that three members of the PAQR family, PAQRs 9, 10, and 11, have a candidate mitochondrial localization signal (MLS) at the N-terminus. We showed that PAQR10 has a functional N-terminal MLS and that the native protein localizes to mitochondria. PAQR10 is structurally related to some bacterial hemolysins, pore-forming virulence factors that target mitochondria and regulate apoptosis. We propose that PAQR10 may act at the level of the mitochondrion to regulate pancreatic endocrine cell development/survival.
Steroid hormones signal through intracellular receptors that translocate to the nucleus and function as transcriptional regulators of target genes. Other steroid-induced events are rapidly triggered independent of transcription via signaling pathways classically associated with cell membrane receptors, including ion channels, second messengers, and protein kinase cascades (1). These so-called nongenomic effects of steroids include estrogen-induced proliferation of breast cancer cells (2), estrogen-induced vasodilation (3), and the progesterone-initiated acrosomal reaction in sperm (4). The mechanisms underlying this rapid signaling by steroids are poorly defined. Studies have identified membrane-associated, steroid-binding proteins in different tissues and species, and there is evidence that some nongenomic steroid effects are mediated by classical G protein-coupled receptors (GPCRs) or involve cytoplasmic activation of the nuclear steroid receptors [reviewed in (5)].
Membrane progestin receptors (mPRs) have recently been identified (6) that belong to a new family of seven-transmembrane proteins termed progestin and adipoQ receptors (PAQRs), present in all species except those in the Archae (7,8). This family includes 11 mammalian genes (PAQR1-11), YOL002c and related genes from Saccharomyces cerevisiae (9), and the gene for hemolysin III from Bacillus cereus (10) as well as hemolysin-related bacterial genes. Phylogenetic analysis (8) allows the mammalian PAQR family to be divided into three main subgroups: the adiponectin-related receptors, which include PAQR 1 (adipoR1), PAQR 2 (adipoR2), PAQR 3, and PAQR 4; the mPRs, which include PAQR 5 (mPRγ), PAQR 6, PAQR 7 (mPRα), PAQR 8 (mPRβ), and PAQR 9; and the hemolysin III-related receptors, PAQR 10 and PAQR 11.
Conflicting evidence exists regarding the membrane topology and subcellular localization of PAQR family members, as well as the mechanisms by which they bind ligands and transduce signals. The mPRs PAQR5, 7, and 8 are thought to have extracellular N-termini similar to classic GPCRs (11), whereas the adiponectin-type receptors PAQR1 and 2 have intracellular N-termini (12). Tang et al. (8) predicted a common type I membrane topology for all members of the PAQR family, with an intracellular N-terminus and extracellular C-terminal domain, but experimental confirmation of these structural predictions is lacking for most PAQR family members. Indeed, more recent studies (13,14) reveal that PAQR7 has both intracellular N- and C-termini and localizes predominantly to the endoplasmic reticulum. The mPRs are proposed to signal as GPCRs (11), whereas adiponectin receptor signaling is not coupled to G proteins but involves activation of AMP kinase and the perixosome proliferator-activated receptor (PPAR)-α (12). Here we describe the structure, tissue, and subcellular localization of PAQR10, following its cloning from mouse pancreatic islet β-cells.
Materials and Methods
cDNA Cloning and Sequencing
The BA12 clone encoding PAQR10 (15) was isolated from a βTC3 cDNA library constructed in the lambdaZAP Express vector (Stratagene, La Jolla, CA, USA). Total RNA was extracted from βTC3 cells using RNAzolB reagent (Tel-Test, Inc., Friendswood, TX, USA). Poly A+ RNA was prepared from total RNA using Poly A Tract mRNA Isolation System (Promega Corp., Madison, WI, USA). Double-stranded cDNA was then synthesized by AMV reverse transcriptase using a RiboClone cDNA synthesis Systems (Promega) and ligated into lambdaZAP Express phagemid vector (Stratagene). The library was packaged using a GIGAPACK II system (Strata-gene) and had a titer of 6 × 108 pfu/mL. Screening by hybridization was performed with the β-cell-specific BA12 DNA originally identified by PCR-based representational difference analysis (15), 32P-labeled with the Megaprime DNA labeling system (Amersham-Pharmacia Biotech, Uppsala, Sweden). After three rounds of screening, insert-containing pBK-CMV plasmids were excised from phage clones and the resulting pBK-BA12 sequenced.
Plasmids were sequenced by the dideoxy-terminator method using a 320A sequenator (Applied Biosystems, Foster City, CA, USA). The BLAST algorithm was used to scan databases for protein and DNA homologies.
Northern Blot Analysis
A mouse multiple tissue Northern blot (BD Biosciences Clontech, Palo Alto, CA, USA) was probed with a 32P-labeled cDNA probe corresponding to full-length BA12. Hybridization was performed for 2 h at 68°C in ExpressHyb Hybridization Solution (Clontech). Filters were washed in 2× saline-sodium citrate (SSC) solution, 0.05% SDS, for 30 min at room temperature followed by 0.1 × SSC, 0.1% SDS for 30 min at 50°C, and exposed to Hyperfilm MP (Amersham Pharmacia Biotech) for 24 h at −70°C.
Tissue Screening by RT-PCR
Mouse tissues were dissected from 6- to 8-wk-old C57Bl/6 mice and washed in ice-cold phosphate buffered saline (PBS); RNA was extracted using RNAzol B reagent. DNase I-treated RNA was reverse transcribed with 200 units MMLV RT (Life Technologies, Invitrogen Corp., Carlsbad, CA, USA) in the presence of 0.5 µM random hexanucleotides (Bresatec, GeneWorks Pty. Ltd., Thebarton, SA, Australia) and 200 µM dNTPs. One-tenth volumes of the first-strand synthesis reactions were amplified by PCR in PCR buffer (Perkin Elmer Inc., Shelton, CT, USA) containing 200 µM dNTPs, 1 unit Taq polymerase, and 1 µM each of sense and antisense oligonucleotide primers specific for PAQR10 (forward primer 5′-CGCGGCGATGTTCACTCTGGCCAG-3′ and reverse primer 5′-CAGGCAGATCTTGGCACAGTTCAC-3′), PAQR1 (forward primer 5′-GGCAATGGGGCTCCTTCTGGTAACA-3′ and reverse primer 5′-GAACGAAGCTCCCCATAATCAGTAG-3′), PAQR7 (forward primer 5′-CACTGGTGGAGGGAAAAGAA-3′ and reverse primer 5′-AGCTGGAAACAGTGTGCAAGA-3′), PAQR9 (forward primer 5′-CGGGTCTCTTCGACATCATT-3′ and reverse primer 5′-TTACTGCAGAATTCGGTGCTG-3′), PAQR11 (forward primer 5′-GATCAATGCGGTTCAGGAAT-3′ and reverse primer 5′-TCTCCCAGCAGTCATCAGACA-3′) and β-actin (forward primer 5′-GTGGGCCGCCCTAGGCACCA-3′ and reverse primer 5′-CTCTTTGATGTCACGCACGATTTC-3′). PCR reactions were performed for 35 cycles (95°C/30 s; 56°C/1 min; 72°C/1 min), and amplified products were analyzed on 1.5% agarose gels.
Plasmid constructs were generated to produce versions of PAQR10 protein tagged either at the N-terminus, the C-terminus, or both. To construct a version of PAQR10 FLAG-tagged at the N-terminus (FLAG-PAQR10), full-length sequences were amplified from pBK-BA12 with a specific forward primer (5′-CCCGAAGCGGATCCTGGTGTT-3′) to introduce a BamHI restriction enzyme site and a reverse primer (5′-GACCCCTCGAGTCTGGGCCAC-3′) to introduce a XhoI site. The PCR product was digested with BamHI and XhoI and ligated into BglII/XhoI-digested pCMV-Tag1 vector (Stratagene).
PAQR10 Myc-tagged at the C-terminus (PAQR10-Myc) was constructed with the same forward primer and a reverse primer (5′-GGCCACTCACTCGAGCACCTTGGT-3′) to introduce a XhoI site. The PCR product was digested with BamHI and XhoI and ligated into pCMV-Tag1 vector similarly digested with BamHI and XhoI. The same PCR product digested with BamHI and XhoI was ligated into BglII/XhoI-digested pCMV-Tag1 vector to generate double-tagged PAQR10 (FLAG-PAQR10-Myc).
PAQR10 tagged with enhanced green fluorescent protein (EGFP) at the N-terminus (GFP-PAQR10) was constructed using the same forward primer and a reverse primer (5′-TCACTGCAGATGTTGCTTTGA-3′) to introduce a PstI site. The PCR product was digested with BamHI and PstI and ligated into BglII/PstI-digested pEGFP-C2 (Clontech). A different reverse primer (5′-CACTCATCTGCAGACCTTGGT-3′), introducing a PstI site, was used to construct PAQR10 EGFP-tagged at the C-terminus (PAQR10-GFP). The PCR product was digested with BamHI and PstI and ligated into BglII/PstI-digested pEGFP-C2 (Clontech).
Cell Culture and Transfection
All culture media were from Invitrogen-Gibco (Carlsbad, CA, USA). SV40-transformed mouse cell lines βTC3 and αTC1 (16) that secrete the hormones insulin and glucagon, respectively, were kindly provided by Dr. Doug Hanahan (University of California, San Francisco, CA, USA). Both cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 25 mM glucose, 10% FCS, and antibiotics under a 10% CO2 atmosphere at 37°C. For transfection, βTC3 cells were seeded at 80% confluency in 6-well plates and transfected with 5 µg DNA/well using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions.
CHO-K1 cells plated at a density of 1 × 106 per well were transfected with 1 µg DNA using Lipofectamine 2000 within 24 h of plating.
FLAG-tagged proteins were detected using an anti-FLAG M2 monoclonal antibody (Sigma-Aldrich, Castle Hill, NSW, Australia). Myc-tagged proteins were detected using anti-Myc-Tag monoclonal antibody (clone 9B11; Cell Signaling Technology, Inc., Beverly, MA, USA). GFP fusion proteins were detected by direct fluorescence in the FITC channel. A rabbit polyclonal anti-prohibitin antibody (RB-292; NeoMarkers, Fremont, CA, USA) was used to determine enrichment in mitochondrial localization assays. Rabbit and rat antisera were generated against a peptide corresponding to amino acid residues 20–36 (NDRVPAHKRYQPTEYEH) of PAQR10. Peptides were synthesized with an extra cysteine residue at the N-terminus and then coupled to diphtheria toxoid (DT) via a maleimidocapoyl-N-hydroxysuccinimide (MCS) linker. Free and coupled peptides were purchased from Mimotopes Pty. Ltd. (Clayton, Victoria, Australia). DT-coupled peptides (0.5 mg) were emulsified with Freund’s complete adjuvant and injected at multiple subcutaneous sites into two rabbits each. Following two booster immunizations 6 and 8 wks later, rabbits were bled and sera stored at −20°C. Rabbit immunoglobulins (IgGs) were purified from antisera by protein G-Sepharose (Amersham-Pharmacia Biotech) affinity chromatography. To produce rat antisera, two Wistar rats were injected intraperitoneally with 50 µg DT-coupled peptide in Freund’s complete adjuvant; after two booster immunizations 4 and 8 wks later, rats were killed and their blood collected and sera stored at −20°C.
Adult C57BL/6 mice were housed under standard 12-h light/dark conditions and provided with food and water ad libitum. All experiments were approved by the Animal Research Ethics Committee, Melbourne Health. For the collection of fetal tissue, timed pregnant mice were killed by CO2 narcosis at gestational times e10.5, e12.5, e13.5, e15.5, and e17.5 d; tissue was also collected from newborn mice. Pancreata dissected from embryos and adult mice were fixed for 2–4 h in 4% paraformaldehyde (PFA) in PBS, dehydrated, and embedded in paraffin. Tissue sections (4 µm) were placed on uncoated glass slides, deparaf-finized in xylene, and rehydrated in graded alcohols. Endogenous peroxidase was blocked by immersion in 0.03% hydrogen peroxide for 15 min. Rat anti-PAQR10 sera were diluted 1:200 in PBS, added to the slides, and incubated for 60 min at room temperature in a humidified chamber, followed by incubation with HRP goat anti-rat secondary antibody (Silenus, Hawthorn, Victoria, Australia) diluted 1:300 in PBS for 30 min at room temperature. The sections were then visualized using 3,3′-diaminobenzidine as chromogen and counterstained with Mayer’s hematoxylin. To confirm the specificity of staining, anti-PAQR10 serum was preincubated for 16 h at 4°C with 20 µg/mL of the immunizing peptide before addition to the slides. Digital images were captured with an Axiocam camera from an Axioplan2 compound microscope (Carl Zeiss, Göttingen, Germany).
Mitochondria were enriched from αTC1 and βTC3 cells by selective permeabilization with low concentrations of digitonin (17). Briefly, cells were harvested by trypsinization, resuspended in ice-cold PBS, collected by centrifugation (800g for 5 min) in microfuge tubes at 1 × 106 cells/sample; cell pellets were resuspended in 100 µL ice-cold lysis buffer (80 mM KCl, 250 mM sucrose, 200 µg/mL digitonin in PBS). After 5 min on ice, samples were centrifuged at 10,000g for 5 min; the supernatants containing mainly cytoplasmic proteins and mitochondria-enriched pellets were recovered. Samples were solubilized in 4× SDS sample buffer before SDS-PAGE in 10% to 20% Trisglycine Novex gels (Invitrogen) and Western blotting.
Transfected CHO-K1 cells were grown to 50% confluency on sterile 18 × 18-mm microscope glass cover slips (Chance Propper Ltd., Smethwick, Warley, England) in 6-well culture plates. Twenty-four hours after transfection, Mitotracker red (Molecular Probes, Inc. Eugene, OR, USA) at a 0.25 nM final concentration was added to the medium for 30 min at 37°C. The cover slips were washed twice in PBS, fixed in 4% paraformaldehyde, and incubated with mouse anti-FLAG and anti-Myc primary antibodies for 60 min at room temperature, followed by sheep anti-mouse IgG conjugated to FITC (Silenus-AMRAD Biotech, Boronia, Victoria, Australia). Confocal images were obtained with a Leica TCS4 SP2 spectral confocal scanner and a Leica DMIRE2 microscope (Leica Microsystems, Gladesville, NSW, Australia) equipped with a 100× oil immersion objective.
We previously reported candidate genes specific for pancreatic β-cells identified by PCR-based representational difference analysis of the mouse pancreatic insulin-producing β TC3 and glucagon-producing αTC1 cell lines (15). One of the PCR-generated sequences (BA12) was used to isolate and sequence a cDNA clone from a β TC3 cDNA library. The isolated BA12 clone contained a single open reading frame encoding a protein of 247 amino acids. A BLAST search of the deduced BA12 protein sequence showed it to be identical to monocyte to macrophage differentiation 2 (Mmd2) factor, which had been identified in a screen for mouse gonad-specific genes (18), and to progestin adipoQ receptor 10 (PAQR 10) annotated in GenBank (accession no. AY424299) as a member of the PAQR family. Initial analysis of the BA12 amino acid sequence using the Predict-Protein program (https://doi.org/cubic.bioc.columbia.edu/predictprotein/) revealed a protein with a predicted molecular mass of 29 kDa, comprising seven transmembrane domains oriented with an intracellular N-terminus and an extracellular C-terminus. This topology was consistent with that predicted by Tang et al. (8) for all members of the PAQR family. The protein also contained a mitochondrial targeting sequence (MTS) at the N-terminus and a predicted cleavage site between Arg at position 28 and Tyr at position 29. Comparison of the mouse and human PAQR10 cDNAs in the UCSC Genome Bioinformatics databases (https://doi.org/genome.ucsc.edu/) revealed that mouse and human PAQR10 genes are encoded by seven exons with similar splicing sites. Mouse PAQR10 maps to chromosome 5qG2 and the human gene to chromosome 7p22.1. These mouse and human chromosomal regions show significant syntenic homology.
Northern blot analysis (Figure 1A) revealed a 2.3-kb PAQR10 transcript strongly expressed in testis and brain and weakly in liver, heart, and kidney. By RT-PCR, we examined expression of PAQR10 in different mouse tissues in comparison to PAQR1, PAQR7, and PAQR11 by RT-PCR (Figure 1B). PAQR1, PAQR7, and PAQR11 were selected as being representative, respectively, of the adiponectin receptor, mPR, and hemolysin III subtypes of PAQR family members. PAQR10 was expressed in pancreas, liver, kidney, small intestine, colon, heart, thymus, and brain, and in βTC3 but not αTC1 cells. In contrast, PAQR1, PAQR7, and PAQR11 were expressed in all tissues, except the pancreas, where PAQR7 and PAQR11 expression was negligible or absent. Expression of PAQR7 and PAQR11 was, however, consistently detected in the βTC3 cell line. In the αTC1 cell line, a single, slightly larger PCR product was observed for PAQR7, consistent with mRNA processing by alternative splicing.
In investigating the expression of PAQR10 in the pancreas during mouse embryonic development, we took advantage of the fact that Ngn3, a basic helix-loop-helix transcription factor, marks the pancreatic endocrine lineage (19). Moreover, gene profiling in e13.5 and e15.5 Ngn3-deficient mice indicated that expression of PAQR10 is dependent on that of Ngn3 (20). PAQR10 expression was mapped in relation to that of Ngn3 and PAQR family members representative of the three subtypes (Figure 2). PAQR10 expression was low in whole embryos at e10.5 and e12.5. In the pancreas, PAQR10 expression was highest at e13.5 and e15.5, when the pancreatic epithelium is undergoing branching morphogenesis and differentiation toward the endocrine lineage, and then progressively decreased in older embryonic (e17.5) and newborn pancreas, when it is presumably restricted to β-cells. This pattern of PAQR10 expression mirrors that of Ngn3. Of the other PAQR family members, PAQR1 and PAQR9 showed consistent expression throughout pancreas development, and PAQR11 was expressed during embryogenesis but was undetectable in the newborn pancreas (Figure 2).
In the embryonic pancreas, expression of PAQR10 protein was restricted to branching epithelial structures at e15.5, being absent from parenchyma (Figure 3A). At e17.5, expression was detected in endocrine islet structures and in the ducts (Figure 3B), a pattern maintained in the newborn (Figure 3C). In the adult pancreas, PAQR10 expression was restricted to islet cells, presumably β-cells, being absent in ducts (Figure 3D). Notably, however, prominent expression was observed in both islets and ducts of the maternal pancreas during pregnancy (e9.5) (Figure 3E). The punctate pattern of cytoplasmic staining of PAQR10 (Figure 3F) suggested localization to mitochondria. We therefore determined if the MLS at the N-terminus of PAQR10 directed the subcellular localization of the protein.
Bioinformatic analysis (Table 1) indicated that of the 11 family members only PAQR 9, 10, and 11 were predicted to localize to mitochondria. For PAQR10, TargetP predicted a mitochondrial targeting sequence of similar length in mouse (29 amino acids) and human (28 amino acids), with an identical cleavage site. To determine if the MTS of PAQR10 was functional, we examined the subcellular localization of endogenous PAQR10 in βTC3 cells and of tagged versions of PAQR10 after overexpression in CHO cells. A 29-kDa band corresponding to PAQR10 was detected by Western blotting in total cell lysates and in enriched mitochondrial fractions of βTC3 but not αTC1 cells (Figure 4A, left panel). Mitochondrial enrichment was confirmed by blotting the cytoplasmic and mitochondrial fractions from these cells with an antibody against the mitochondrial protein, prohibitin. A 30-kDa band corresponding to prohibitin was detected only in mitochondrial fractions (Figure 4A, right panel). The localization of tagged versions of PAQR10 transfected into CHO cells was then investigated by immunofluorescence and confocal microscopy (Figure 4B). Mitochondria were labeled by exposure of transfected CHO cells to Mitotracker dye. The C-terminal tagged versions of the protein, either PAQR-myc or PAQR-GFP, localized to mitochondria. Mitochondrial localization was absent if the protein was N-terminally tagged with FLAG or GFP, after which it was observed in perinuclear sites. These results indicated that the MTS at the N-terminus of PAQR10 directs its localization to mitochondria.
We identified PAQR10, a member of the highly conserved PAQR gene family, in a screen for genes differentially expressed in pancreatic β-cell versus a-cell lines. Compared with other PAQR genes, the expression of PAQR10 was more restricted and in adult mouse pancreas was confined to islets. This was confirmed by staining for PAQR10 protein, which was localized to pancreatic ducts and endocrine tissue in the embryo, neonate, and pregnant adult, but to islets only and not ducts in the nonpregnant female. Consistent with this endocrine localization, expression of PAQR10 in the embryo appeared to mirror that of Ngn3, a transcription factor that marks the endocrine lineage. Petri et al. (20) reported that PAQR10 was one of many genes not expressed in Ngn3-knockout mice, which fail to develop an endocrine pancreas. Its expression pattern infers a role for PAQR10 in endocrine pancreas development and hyperplasia in pregnancy.
The potential function of PAQR10 is suggested by its localization to the mitochondrion, which has recently been recognized as a primary site of action of steroid and thyroid hormones (21). Mitochondrial localization is predicted for PAQR9 and PAQR11. PAQR9 is a putative mPR, whereas PAQR10 and 11 are closely related to bacterial hemolysins. Hemolysin III functions as a pore-forming membrane protein (10), and bacterial virulence factors have recently been shown to target mitochondria and up- or downregulate apoptosis in target cells (22). In eukaryotes, pro- and anti-apoptotic members of the Bcl-2 family regulate the mitochondrial pathway of apoptosis by controlling permeability of the outer mitochondrial membrane (23). Because PAQR10 targets mitochondria, and similar targeting is predicted for PAQR11, it will be of interest to determine if these PAQRs, structurally related to hemolysins, have pore-forming, apoptosis-regulating properties, and their relationship to the function of Bcl-2 family members. We suggest that PAQR10 may promote growth-survival of the endocrine pancreas via an effect on the mitochondrial pathway of apoptosis. The ligand for PAQR10 is unknown, but the receptor could act as a signal transducer that translocates to mitochondria upon ligand binding. There are precedents for translocation of signaling molecules between the cell surface and mitochondria: the C1q receptor, a globular protein structurally related to adiponectin, contains a functional MTS at its N-terminus and traffics between the cell surface and mitochondria (24); the receptor for stanniocalcin, a hormone which regulates calcium and phosphate excretion in the kidney and gut, resides in both the plasma membrane and mitochondria (25).
In pregnancy, in response to an increased demand for insulin, pancreatic β-cells undergo major changes to compensate for systemic insulin resistance. These include increases in cAMP metabolism and glucose oxidation, gap junction coupling between β-cells, glucose-stimulated insulin release, insulin synthesis, and β-cell proliferation (26–28). In rodents, maternal β-cell proliferation in pregnancy is induced by the lactogenic hormones, prolactin (PRL) and placental lactogen (PL), and counter-regulated by the steroid hormone progesterone (29). Mice with deletion of the classic progesterone receptor have increased insulin secretion and glucose clearance associated with an increase in β-cell proliferation and mass (30), consistent with an inhibitory effect of progesterone on β-cell proliferation and function. The function of mPRs in the pancreas is unknown, but progesterone has been shown to inhibit insulin secretion directly, by a cell membrane-initiated, nongenomic effect that decreases Ca2+ influx (31). Three PAQR family members, PAQR5, 7, and 8, specifically bind progestins (6,11), and because of sequence similarities, PAQR6 and 9 are also putative mPRs (8). It would be of interest therefore to determine the contribution of PAQR family mPRs to the inhibitory effect of progesterone on β-cell proliferation and function. In regard to PAQR10, however, there is no evidence currently that the ligand for this receptor is a progestin, and our findings suggest that it may not be. Thus, PAQR10 expression mirrored pancreatic endocrine development in the late embryo-neonate and was detected not only in pancreatic islets but also ducts, from which β-cells are known to derive, in pregnancy. These findings are not in keeping with the inhibitory effect of progesterone on β-cell proliferation and function, but rather with promotion of β-cell development and survival.
Finally, the localization of PAQR10 expression to β-cells raises the possibility that it may be a diabetes susceptibility gene. Susceptibility to type 2 diabetes maps to chromosomal regions containing genes for the adiponectin receptors PAQR1 and PAQR2 (32), and family studies show significant associations between adiponectin (33) and PAQR1 (34) polymorphisms and type 2 diabetes. A genome-wide scan for type 2 diabetes genes in Japanese sib pairs (35) identified a region of strong linkage at 7p21-22, with a maximum LOD score at marker D7S517, which is just 0.5 Mb away from PAQR10. We conclude that further studies are likely to establish a key role for PAQR10 in pancreatic β-cell biology.
The authors declare that they have no financial or other conflict of interest relating to the work described in this manuscript.
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This work was supported by a partnership Program Grant from the Juvenile Diabetes Research Foundation (JDRF) and the National Health and Medical Research Foundation of Australia (NHMRC). L.C.H. is a Senior Principal Research Fellow of the NHMRC. L.J.G. was supported in part by a grant from Diabetes Australia Research Trust. The authors thank Catherine McLean for secretarial assistance.
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Góñez, L.J., Naselli, G., Banakh, I. et al. Pancreatic Expression and Mitochondrial Localization of the Progestin-AdipoQ Receptor PAQR10. Mol Med 14, 697–704 (2008). https://doi.org/10.2119/2008-00072.Gonez