- Original Articles
- Published:
Elevated Expression of Transforming Growth Factor-β in Adipose Tissue from Obese Mice
Molecular Medicine volume 3, pages 37–48 (1997)
Abstract
Background
Tumor necrosis factor-α (TNF-α) is chronically elevated in the adipose tissue from obese humans and mice. This increase in TNF-α contributes to the insulin resistance, elevated plasminogen activator inhibitor-1 (PAI-1) levels, and cardiovascular complications associated with obesity and noninsulin-dependent diabetes (NIDDM). PAI-1 gene expression in adipose tissue is also stimulated by transforming growth factor-β (TGF-β). Experiments were performed to determine whether TGF-β is regulated by TNF-α and elevated in obesity.
Materials and Methods
The concentration of TGF-β and PAI-1 mRNA in murine adipose tissue and cultured 3T3-L1 adipocytes was determined by quantitative reverse transcription polymerase chain reaction (RT-PCR), and the cellular localization of these molecules was evaluated using in situ hybridization and cell fractionation. Total TGF-β protein was determined by employing an ELISA assay.
Results
TGF-β mRNA and protein were increased in the adipose tissue from two different strains of genetically obese mice (i.e., ob/ob and db/db), compared with their lean counterparts. This increase in TGF-β may result from TNF-α since TNF-α increased TGF-β mRNA expression in the adipose tissue of lean mice and stimulated TGF-β production by cultured adipocytes. Administration of TGF-β increased PAI-1 antigen in the plasma and PAI-1 mRNA in the adipocytes of lean mice, and enhanced the rate of PAI-1 synthesis by adipocytes in vitro.
Conclusions
TNF-α contributes to the elevated TGF-β expression demonstrated in the adipose tissue of obese mice. A potential role for TGF-β in the increased PAI-1 and vascular pathologies associated with obesity/NIDDM is suggested.
Introduction
Obesity is a risk factor for the development of cardiovascular disease (1,2) and is associated with metabolic disorders such as hypertriglyceridemia, hyperinsulinemia, and noninsulin-dependent diabetes (NIDDM) (2,3). Interestingly, a number of clinical studies also have demonstrated a correlation between obesity and elevated plasma PAI-1 (4–7). PAI-1 is the primary physiological inhibitor of plasminogen activation in vivo (8,9), and increases in plasma PAI-1 are also associated with increased risk for cardiovascular disease (10). Although the importance of the link between obesity and elevated PAI-1 has long been recognized, the molecular basis of this connection remains elusive. In this regard, surgical treatments that cause rapid weight loss in obese individuals lead to a rapid reduction in plasma PAI-1 activity and clot lysis time (11). This observation suggests that the adipose tissue itself may produce PAI-1 and/or cytokines that regulate PAI-1 gene expression in vivo. This hypothesis is supported by the findings that plasma PAI-1 is increased 4- to 5-fold in genetically obese mice when compared with their lean counterparts, and that PAI-1 mRNA is elevated in the adipose tissues of these mice (12).
Studies of obese rodents and humans demonstrate that tumor necrosis factor-α (TNF-α) is elevated in the adipose tissue and that expression of TNF-α is strongly correlated with insulin resistance and hyperinsulinemia (13,14). Interestingly, TNF-α stimulates PAI-1 expression in a variety of cultured cells and in most murine tissues (15,16). In obese humans, elevations in plasma PAI-1 also appear to correlate with high plasma insulin levels (17), and PAI-1 levels have been shown to be directly correlated with insulin resistance (5,18,19). These observations raise the possibility that the elevated levels of plasma PAI-1 in obesity may arise from increased synthesis of PAI-1 by the adipose tissue in response to TNF-α and/or insulin. In this regard, we have demonstrated that direct administration of insulin or TNF-α to lean mice stimulates PAI-1 gene expression in the adipose tissue (12,20,21).
In addition to TNF-α and insulin, the major inducer of PAI-1 in the adipose tissue appears to be transforming growth factor-β (TGF-β) (21). In this study, we investigate whether TGF-β itself is regulated in obesity. We demonstrate that TGF-β gene expression is elevated in the adipose tissues from genetically obese mice, and that its expression in vivo and in vitro is regulated by TNF-α. We also show that TGF-β induces PAI-1 mRNA in the adipocytes in vivo and in vitro. These results suggest that TGF-β may contribute to the various pathologies associated with obesity and NIDDM, and it may play a significant role in the regulation of PAI-1 in obesity.
Materials and Methods
Animals and Tissue Preparation
Adult male CB6 mice (BalbC/ByJ X C57BL6/J), weighing 25–30 g, were obtained from the Scripps Rodent Breeding Colony. Adult male obese mice (C57BL/6J ob/ob; C57BL/KsJ/db/db), aged 8–12 weeks, and their lean counterparts (C57BL/6J +/?; C57BL/KsJ +/?) were obtained from Jackson Labs (Bar Harbor, ME). CB6 mice were injected intraperitoneally with the indicated amount of human recombinant TGF-β (Sigma Chemical Company, St. Louis, MO) dissolved in 4 mM HCl containing 0.1% BSA, or with recombinant murine TNF-α (4 µg/mouse in saline; kind gift of Dr. Richard Ulevitch, The Scripps Research Institute). Control mice were injected with an equivalent amount of 4 mM HCl/0.1% BSA or saline, respectively. For in vivo insulin experiments, lean mice (C57BL/6J +/?) were injected intraperitoneally with 10 units of regular human insulin (Humilin R; Eli Lilly, Indianapolis, IN), while the controls were injected with an equivalent volume of saline alone. At the conclusion of each experiment, mice were anesthetized by metofane (Pitman-Moore, Mundelein, IL), blood samples were collected into 20 mM EDTA (final concentration), and epididymal fat pads were removed and processed for in situ hybridization analysis and preparation of total RNA as previously described (20).
Determination of PAI-1 Activity in Plasma
Active PAI-1 antigen in plasma was determined using the t-PA binding assay as previously described (22). The polyclonal rabbit anti-mouse PAI-1 antibody used in this assay was prepared against purified, recombinant mouse PAI-1. Recombinant mouse PAI-1 was a kind gift from Drs. D. Lawrence and D. Ginsburg, Howard Hughes Medical Institute, University of Michigan Medical Center, Ann Arbor, Michigan. In this assay, active PAI-1 levels (ng/ml) were calculated from a standard curve constructed using recombinant mouse PAI-1.
Quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR)
The concentrations of PAI-1 and TGF-β mRNA were determined by quantitative RT-PCR using a competitor cRNA containing upstream and downstream primers for PAI-1, TGF-β, and β-Actin as previously described (23,24). After reverse transcription (using either 106 or 107 molecules of cRNA for PAI-1, 105 molecules for TGF-β, and 107 molecules for β-actin, optimized in previous preliminary experiments) and PCR (25), 20 µl of the PCR products were electrophoresed on 1.8% agarose gels. The appropriate bands corresponding to the internal standard cRNA product and the target mRNA product were excised from the gel and the incorporated radioactivity quantified using a scintillation counter. A standard curve for the internal control cRNA was constructed and used to determine the specific activity of the target mRNA as previously described (20,24). Variations in sample loading were assessed by measuring β-actin mRNA.
Riboprobe Preparation and In Situ Hybridization
A subclone of mouse PAI-1 cDNA containing nucleotides 1–1085 in the vector pGEM-3Z was used to prepare a riboprobe for in situ hybridization (26). This vector was linearized with EcoRI (antisense) or HindIII (sense) and used as a template for in vitro transcription of radiolabeled antisense or sense riboprobes employing SP6 or T7 RNA polymerase, respectively, in the presence of [35S]UTP (>1,200 Ci/mmol; Amersham Corp., Arlington Heights, IL). A 974 bp fragment of the mouse TGF-β cDNA (kindly provided by Dr. Rik Derynck, Genentech, Inc; [27]) was subcloned into the vector pSP73 and used to generate TGF-β-specific riboprobes. In situ hybridization was performed as described previously using the above 35S-labeled riboprobes (26). Both sense and antisense probes were routinely labeled to specific activities between 0.5 and 2 × 108 cpm/µg RNA. Slides were exposed in the dark at 4°C for 4 to 12 weeks.
Tissue Digestion and Cell Fractionation
Epididymal fat pads were isolated from mice, washed in sterile PBS, minced, and washed in Krebs-Ringer Bicarbonate (KRB) buffer (pH 7.4) containing 4% albumin and 5 mM glucose (28). The tissues were then treated with collagenase (2 mg/ml; Sigma) on a shaking platform at 37°C for 1 hr. Undigested tissue was removed with forceps, and the adipocytes were then separated from other cells by their ability to float upon low-speed (200g) centrifugation. To obtain the total stromal-vascular fraction, the medium below the adipocyte layer was centrifuged at 500g for 10 min, and the pellet was collected and washed three times with warm KRB buffer. Total RNA was extracted from the two fractions and the amount of PAI-1 or TGF-β mRNA associated with each was determined by quantitative RT-PCR as described above.
TGF-β Protein Production by Adipose Tissue
Local TGF-β protein production over 24 hr was determined in explanted adipose tissues from 6-month-old ob/ob mice and their lean counterparts. Epididymal fat pads were dissected under sterile conditions, weighed, and then rinsed first in sterile phosphate-buffered saline (PBS) and then in sterile Krebs-Ringer bicarbonate (KRB) buffer (pH 7.4) containing 4% serum albumin. The tissue was minced and incubated in a medium containing Dulbecco’s modified Eagle’s/Ham’s F-12 supplemented with 10% FBS; 5 mM glutamine; penicillin (6.35 mg/ml); and streptomycin (5 mg/ml) in 6-well tissue culture plates at 37°C. The conditioned medium was collected after 24 hr and total TGF-β protein (i.e., after acid activation) was determined using the PREDICTA ELISA assay (Genzyme, MA). Since fetal bovine serum (FBS) used for the above cultures may contain TGF-β, an organ culture medium control was activated and run in the assay. At the end of the experiment, total DNA was extracted from the epididymal fat pads used in each experiment as described (29), and TGF-β values expressed both as ng TGF-β/mg of DNA and ng TGF-β/g of tissue.
Cell Culture
3T3-L1 mouse embryo fibroblasts were obtained from the American Type Culture Collection. The culturing of these cells and their differentiation from preadipocytes into mature adipocytes was carried out as described previously (30). Total RNA was isolated from untreated mature adipocytes, and from mature adipocytes treated with insulin (bovine insulin, Sigma), TNF-α, or TGF-β as indicated. Conditioned medium was collected from untreated and treated adipocytes for determination of PAI-1 antigen levels.
Western Blot Analysis
Conditioned medium (20 µl) from untreated and TGF-β-treated adipocytes was electrophoresed under nonreducing conditions on a 9% SDS-PAGE gel and transferred to PROTRAN nitrocellulose membranes (Schleicher & Schuell, Keene, NH). PAI-1 antigen was detected using a polyclonal rabbit antiserum specific for murine PAI-1, and the ECL system (Amersham) as previously described (12).
Results
Tissue Distribution and Cellular Localization of TGF-β mRNA in Lean and Obese Mice
Previous studies showing that TGF-β stimulates PAI-1 gene expression in the adipose tissues of normal CB6 mice (21), combined with the demonstration that PAI-1 is elevated in obesity (12), prompted us to more closely examine the role of TGF-β in obesity. We initially determined whether TGF-β expression was up-regulated in tissues from obese mice. Various tissues were removed from lean and obese mice and total RNA was prepared and analyzed for TGF-β mRNA by quantitative RT-PCR. Figure 1A shows that TGF-β mRNA levels were approximately 2-fold higher in the adipose tissues of 3-month-old ob/ob mice compared with their lean counterparts, and 5-fold higher in 6-month-old ob/ob mice. This age-dependent increase in TGF-β mRNA was not observed in the lean animals. TGF-β mRNA expression was also increased (3- to 4-fold) in adipose tissue from the db/db mouse (Fig. 1A), another murine model of obesity. Although TGF-β mRNA was slightly elevated in muscle from 3-month-old obese mice, it was unchanged in the kidney, heart, brain, lung and liver (data not shown).
Expression of TGF-ß mRNA in adipose tissues from lean and obese mice.
(A) Total RNA was extracted from epididymal fat pads of male lean and obese (ob/ob; db/db) animals of the indicated ages. TGF-β mRNA was determined using quantitative PCR analysis, n = 6 ± SD. (B) Cell fractionation of adipose tissue from lean and obese mice. Epididymal fat pads were isolated from 6-month-old male lean and ob/ob mice. Adipocytes (AD) and stromal vascular cells (S/V) were separated by collagenase digestion followed by differential centrifugation. Total RNA was extracted from each fraction and TGF-β mRNA levels were quantified by RT-PCR. n = 3.
Experiments were performed to determine whether the increased TGF-β mRNA in adipose tissue from obese mice is associated with increased production of TGF-β protein. Local TGF-β production was determined in explanted epididymal fat pads from 6-month-old ob/ob and lean mice. The obese and lean epididymal fat pads (n = 6) produced 1.15 ± 0.4 and 0.33 ± 0.17 ng TGF-β/g of tissue, respectively. When expressed as the mass of TGF-β produced per unit of tissue DNA, the obese and lean adipose tissues produced 6.9 ± 1.6 and 2.27 ± 1.7 ng TGF-ß/mg DNA, respectively. Although there was a 3- to 4-fold increase in the local production of TGF-β protein from the obese adipose tissue, there were no significant differences in the circulating plasma levels of TGF-β protein (i.e., 6.3 ± 1.56 ng/ml total TGF-β in ob/ob mice versus 4.9 ± 1.8 ng/ml total TGF-β in the lean). These data suggest that TGF-β produced in the adipose tissue may function locally via autocrine and paracrine mechanisms.
Experiments were performed to identify the cells in adipose tissue expressing TGF-β mRNA. Adipose tissues from lean and obese (ob/ob) mice were dissociated by collagenase treatment and subjected to differential centrifugation to separate lipid-laden mature adipocytes (floating cells) from the stromal/vascular (pellet) cells as described (28). The purity of the mature adipocyte fraction was documented by the failure to detect endothelial cells in this fraction by RT-PCR (data not shown) using primers specific for von Willebrand factor as previously described (20). Figure 1B shows that TGF-β mRNA levels were increased in both the mature adipocyte and the stromal/vascular cell fractions of the adipose tissues from the obese mice.
Adipose tissue contains a variety of cells besides adipocytes, including preadipocytes, vascular endothelial and smooth muscle cells, fibroblasts, local mast cells, and macrophages (31). Thus, in situ hybridization experiments were performed to further identify the TGF-β-expressing cells (Fig. 2). A weak, but consistent signal for TGF-β mRNA was detected in cells in the fat from lean mice (Fig. 2A). However, a much stronger signal was associated with these cells in the fat from obese mice (Fig. 2B-D). The differences in the shapes of the nuclei of the positive cells and their different location, together suggests that TGF-β mRNA is expressed by multiple cell types. For example, red blood cells (RBCs) are observed under the signal in Fig. 2C, suggesting that capillary endothelial cells express TGF-β mRNA. The observation that the hybridization signal is also localized outside of the capillaries (Fig. 2C, D) suggests that adipocytes also synthesize TGF-β, which is consistent with the results shown in Fig. 1B. No signal for TGF-β was detected in the smooth muscle cells or endothelial cells of the larger vessels in the adipose tissue of the lean or obese mice (data not shown). In control experiments, no specific hybridization signal was detected using a sense TGF-β riboprobe (data not shown).
Cellular localization of TGF-β mRNA expression in adipose tissue from lean (+/?) and obese (ob/ob) mice.
Representative sections showing the cellular localization of TGF-β mRNA. In situ hybridization was performed on sections of paraffin embedded adipose tissues from lean (A, ×400) and obese (B, ×400 C, D, ×1000) mice. a, adipocyte; rbc, red blood cell. Arrows indicate positive hybridization signal in adipocytes (B and D) and capillary cells (C). Slides for in situ hybridization were exposed for 8 weeks at 4°C and stained with hematoxylin and eosin.
Effect of TNF-α and Insulin on TGF-β Gene Expression in Adipose Tissues and in Cultured 3T3-L1 Adipocytes
Since TNF-α and insulin are elevated in obesity (3,13,14), we determined whether these agents could regulate the expression of TGF-β in the adipose tissue. Lean CB6 mice were injected intraperitoneally with either 4 µg of TNF-α or 10 units of insulin, or with vehicle alone. Tissues were removed 3, 6, and 24 hr later, and total RNA was prepared and analyzed for TGF-β mRNA by quantitative RT-PCR (Fig. 3A). TNF-α transiently increased TGF-β mRNA expression in the adipose tissues with a maximum induction of 6- to 8-fold by 6 hr, and a return to basal levels by 24 hr of treatment. Insulin did not appear to induce TGF-β mRNA expression in the adipose tissue under these conditions.
Induction of TGF-β mRNA expression by insulin and TNF-α.
(A) Six-week-old lean mice were injected intraperitoneally either with 10 units of insulin, with 4 µg of murine recombinant TNF-α, or with saline. The epididymal fat pads were removed 3, 6, and 24 hr later and total RNA was prepared and analyzed for TGF-β gene expression by quantitative RT-PCR as described in Materials and Methods. Each time point on the graph represents the mean ± SD of 3 animals. (B) 3T3-L1 adipocytes were grown and differentiated in 6-well tissue culture plates. The washed and differentiated cells were then treated with insulin (100 nM), TNF-α (5 ng/ml), or media alone for the indicated times, and total RNA was isolated and the steady-state levels of TGF-β mRNA were determined using quantitative RT-PCR. n = 6 ± SD.
In vitro experiments also were performed to examine the regulation of TGF-β gene expression in adipocytes. Figure 3B shows that treatment of 3T3-L1 adipocytes with TNF-α (5 ng/ml) again increased TGF-β mRNA expression, with maximum induction at 24 hr and a decline to baseline values by 48 hr. The difference in the kinetics of the in vivo (Fig. 3A) and in vitro (Fig. 3B) response may reflect the different half lives of TNF-α in vivo and under cell culture conditions. Although insulin did not seem to induce TGF-β mRNA expression in the adipose tissue in vivo (Fig. 3A), it caused a modest and transient increase in TGF-β when added to cultured 3T3-L1 adipocytes in vitro (Fig. 3B).
Effect of TGF-β on PAI-1 Expression
We previously showed that TGF-β induced PAI-1 mRNA in the adipose tissue in vivo (21). However, in those experiments, neither the identity of the responsible cells nor the effect of TGF-β on plasma PAI-1 was determined. Figure 4A shows that TGF-β also induced active PAI-1 in the plasma, increasing it from a basal level of 1.2 ± 0.05 ng/ml to 61.0 ± 2.5 ng/ml by 3 hr. Thus, the induction of PAI-1 mRNA by TGF-β is associated with a concurrent increase in active PAI-1 antigen in plasma.
Plasma PAI-1 activity and PAI-1 mRNA expression in cell-fractionated adipose tissue after TGF-β treatment.
(A) Six- to eight-week-old male lean CB6 mice were injected intraperitoneally with 2 µg human recombinant TGF-β or diluent. The animals were sacrificed 3 hr later and blood was collected into 20 mM EDTA (final concentration). After centrifugation, the plasma was collected and active PAI-1 (ng/ml) was determined using the t-PA binding assay. (B) Epididymal fat pads were isolated from 6- to 8-week-old CB6 mice 3 hr after intraperitoneal injection of TGF-β (2 µg). Mature adipocytes (AD) and stromal vascular cells (S/V) were separated by differential centrifugation and total RNA was prepared and PAI-1 mRNA levels were determined using RT-PCR. n = 3, ± SD.
Cell fractionation experiments were performed to begin to identify the cell-type(s) expressing elevated PAI-1 levels after TGF-β treatment. Adipose tissue was again subjected to differential centrifugation to separate mature adipocytes from the stromal/vascular cells, and the concentration of PAI-1 mRNA in the two cell fractions was determined by RT-PCR. Figure 4B shows that TGF-β induced PAI-1 mRNA both in the mature adipocyte fraction and in the stromal/vascular cell fraction. In situ hybridization experiments demonstrate a weak, but consistent signal for PAI-1 mRNA in the smooth muscle cell layer within arteries in the adipose tissue from untreated mice (Fig. 5A). This signal was increased significantly in TGF-β-treated mice (Fig. 5B). Interestingly, in both instances, endothelial cells were negative for PAI-1 mRNA (Fig. 5A, B). Although no PAI-1 mRNA was detected by in situ hybridization in the fat from untreated mice (Fig. 5C), a strong positive signal was observed in the fat from TGF-β-treated mice (Fig. 5D). The PAI-1-positive cells morphologically (Fig. 5D) and biochemically (Fig. 4B) resembled adipocytes. Again, in control experiments, no specific hybridization signal was detected using a sense PAI-1 riboprobe (data not shown).
Effect of TGF-β on the cellular localization of PAI-1 mRNA in the adipose tissue of CB6 mice.
In situ hybridization was performed on paraffin sections of epididymal fat pad from untreated mice (A and C) and mice treated with TGF-β (2 µg) for 3 hr (B and D). Slides were exposed for 8 weeks at 4°C and stained with hematoxylin and eosin. e, endothelial cells; sm, smooth muscle cells; a, adipocytes. Arrowheads indicate positive signal for PAI-1 mRNA in the fat (D). Magnification was ×400 for all sections.
Effect of TGF-β on PAI-1 Expression in Cultured 3T3-L1 Adipocytes
PAI-1 synthesis by 3T3-L1 adipocytes is induced by TNF-α (20) and insulin (12), two mediators that are elevated in obesity. Thus, experiments were performed to determine whether PAI-1 expression by these cells also was induced by TGF-β. Figure 6A shows that TGF-β rapidly but transiently increased PAI-1 mRNA expression in these cells, with a maximum induction of 10- to 15-fold within 3 hr, and a return to basal levels by 24 hr. PAI-1 antigen in the conditioned medium was also induced by TGF-β (Fig. 6A; inset). The 3T3-L1 cells responded to TGF-β in a dose-dependent manner (Fig. 6B), with an increase of 10- to 12-fold at the maximum dose employed (1 ng/ml). The increase in PAI-1 mRNA paralleled the induction of PAI-1 antigen (inset).
Stimulation of PAI-1 expression in 3T3-L1 adipocytes by TGF-β.
3T3-L1 adipocytes were grown and differentiated in 6-well tissue culture plates as described previously (28). For kinetic studies (A), total RNA was isolated from untreated cells (●), and cells treated with 1 ng/ml TGF-β (■) for the indicated times, and the steady-state levels of PAI-1 mRNA were determined using quantitative PCR. Inset: Conditioned medium (20 µl) from untreated cells and cells treated with TGF-β for various times were electrophoresed under nonreducing conditions on a 9% SDS-PAGE gel and the proteins were transferred to nitrocellulose membranes. The membranes were analyzed for PAI-1 antigen by Western blotting using a polyclonal rabbit anti-mouse PAI-1 antiserum and the enhanced chemiluminescence detection system. For dose-response experiments (B), total RNA was isolated from untreated cells, and cells treated with increasing amounts of TGF-β for 3 hr. Steady-state levels of PAI-1 mRNA were determined as above. Inset: Western blot analysis for PAI-1 antigen in conditioned medium (20 µl) from untreated cells, and cells treated with increasing amounts of TGF-β for 24 hr. For both A and B, n = 6 ± SD.
Discussion
TGF-β is a multifunctional cytokine that is produced by a variety of cells and is capable of regulating the growth and differentiation of many cell types (32). It has been implicated in a number of biological processes including cell adhesion and migration, extracellular matrix production, tissue remodeling, and wound repair (32,33). In vivo, TGF-β is present in platelets (33), peripheral blood monocytes, and tissue macrophages (34), and is released from these cells when they are activated. In vitro studies have shown that TGF-β stimulates PAI-1 biosynthesis by a variety of cells, including endothelial cells (35), smooth muscle cells (36), fibroblasts (37), epithelial cells (38), and several transformed cells lines, including hepatoma cells (39). Infusion of TGF-β into rabbits increased plasma PAI-1 activity (40), and infusion into mice induced PAI-1 mRNA in numerous tissues, the largest increase occurring in the adipose tissue (21). These in vitro and in vivo results suggest that TGF-β is a potent regulator of PAI-1 gene expression in adipose tissue. Moreover, they raise the possibility that if TGF-β is elevated in obesity, it may stimulate PAI-1 gene expression and thus contribute to increased plasma PAI-1 associated with this condition.
To evaluate whether TGF-β was elevated in obesity, we determined the concentration of TGF-β mRNA in the adipose tissue of lean and obese mice. TGF-β mRNA levels were significantly higher in the adipose tissue of both the ob/ob and db/db mice when compared to their lean counterparts (Fig. 1A). Moreover, this increase was due to increased expression of TGF-β mRNA by mature adipocytes and unidentified cells in the stromal/vascular fraction (Fig. 1B). In situ hybridization experiments demonstrated a TGF-β hybridization signal in multiple cell types, including adipocytes (Fig. 2B-D). Microvascular endothelial cells may express TGF-β mRNA since a positive signal was observed in cells within some capillaries (Fig. 2C). This possibility is under further investigation. Interestingly, TGF-β mRNA was not detected in large vessels in adipose tissue from lean or obese mice (data not shown).
Experiments were performed to identify potential mechanisms that contribute to the chronically elevated levels of TGF-β associated with the adipose tissues of obese mice. Both the ob/ob and db/db mice are insulin resistant and hyperinsulinemic (41). Moreover, TNF-α is chronically elevated in the adipose tissue of insulin resistant obese rodents and humans (13,14), and TNF-α can induce TGF-β induction in rat endothelial cells and thyroid cells (42). We thus asked whether TGF-β expression in the adipose tissue could be induced by either TNF-α or insulin. TNF-α induced TGF-β mRNA in the adipose tissue of normal CB6 mice (Fig. 3A) and caused a strong induction of TGF-β mRNA in cultured 3T3-L1 cells (Fig. 3B). These observations suggest that the chronically elevated TNF-α levels in the obese adipose tissue may act in an autocrine/paracrine manner and contribute to elevated TGF-β mRNA expression in obesity. Insulin failed to increase TGF-β mRNA in adipose tissues of lean mice (Fig. 3A), even though it significantly increased the expression of PAI-1 mRNA in adipose tissue (12). The addition of insulin to cultured 3T3-L1 cells caused a modest elevation of TGF-β mRNA.
The increase in TGF-β gene expression in adipose tissue in obesity may have broad implications in the pathophysiology of obesity and its related complications. TGF-β inhibits in vitro proliferation of many cells, but it stimulates the growth of fibroblasts (43,44). Moreover, TGF-β has been shown to increase preadipocyte cell proliferation in many species (45–48). Thus, the augmented expression of TGF-β in the obese adipose tissue may increase adipocyte precursor cell proliferation, thereby contributing to the excessive cellularity of the fat depots associated with the obese phenotype. Obesity and NIDDM are also associated with characteristic long-term complications, including microvascular kidney disease (49,50) and atherosclerosis (51,52). It is interesting to note in this regard that several investigators have reported overexpression of TGF-β in the glomeruli in human and experimental diabetes (50,53). Although TGF-β expression was not elevated in the kidney of 3-month-old ob/ob mice, a 2- to 3-fold increase in TGF-β mRNA was observed in the kidney of 6-month-old ob/ob mice, in comparison with its lean counterpart (data not shown).
Obesity and NIDDM are also associated with elevated PAI-1 (4–7), and administration of TGF-β to lean mice caused a 60-fold increase in active PAI-1 in plasma and increased PAI-1 mRNA in adipose tissue (Fig. 4). Cell fractionation studies (Fig. 4B) and in situ hybridization analysis (Fig. 5) of adipose tissues from TGF-β-treated mice demonstrated that TGF-β induced PAI-1 mRNA expression in mature adipocytes and in the vascular smooth muscle cells of the arteries within the adipose tissue. TGF-β did not induce PAI-1 mRNA expression in large vessel endothelial cells in the adipose tissue, which is in agreement with previous studies in the kidney (26). TGF-β also stimulated PAI-1 gene expression by cultured 3T3-L1 cells (Fig. 6), confirming the recent studies of Lundgren et al. (54). The TGF-β-mediated induction of PAI-1 in 3T3-L1 adipocytes was considerably higher than the response of these cells to TNF-α and insulin (12,20). Similarly, the greatest PAI-1 response in the adipose tissue in vivo was to TGF-β (36-fold; [21]) followed by TNF-α, (9-fold; [21]) and insulin (7-fold; [12]).
The mechanisms that lead to the elevated plasma PAI-1 levels observed in obesity and related NIDDM are obviously complex and may involve multiple cytokines, hormones, and growth factors. The fact that TNF-α (13,14), insulin (41), and TGF-β (this study) are elevated in obesity and induce PAI-1 in the plasma and adipose tissue of lean mice (20) (Fig. 4) certainly suggests the involvement of at least these three mediators in the regulation of PAI-1 in obesity. However, the relationship between these mediators and the elevated levels of PAI-1 is at present only correlative. Direct evidence would involve further experiments employing specific inhibitors or neutralizing antibodies. Obesity is associated with an excess of adipose tissue that frequently includes an elevated number of fat cells (55). This situation would tend to exacerbate the overproduction of TGF-β, PAI-1, and other molecules, whether the effects are achieved locally or systemically. These considerations emphasize the potential importance of excess adipose tissue mass for the synthesis of proteins and other molecules that may promote the development of pathophysiological complications associated with obesity.
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Acknowledgments
This work was supported by grant HL 47819 to DJL. The authors thank T. Thinnes for her technical assistance. In addition we thank M. McRae for her expert secretarial skills.
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Samad, F., Yamamoto, K., Pandey, M. et al. Elevated Expression of Transforming Growth Factor-β in Adipose Tissue from Obese Mice. Mol Med 3, 37–48 (1997). https://doi.org/10.1007/BF03401666
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DOI: https://doi.org/10.1007/BF03401666